Because there are over a dozen different plug configurations for the 2UZ-FE, you should verify your ECU part number (e.g., ) against a factory service manual.
Most 2UZ-FE ECUs from 2000 onwards are "locked" to a specific transponder key. If you don't have the original key and the amplifier ring, the engine will start for 2 seconds and die. You may need an Immobilizer Bypass or a "virginized" ECU. 2uzfe ecu pinout
Ignition triggers for the Coil-on-Plug (COP) units. Because there are over a dozen different plug